4-AcO-DMT

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4-AcO-DMT (4-acetoxy-N,N-dimethyltryptamine), also known as O-acetylpsilocin, is a semi-synthetic tryptamine first synthesized by Albert Hofmann in 1963. It is closely related to psilocin, sharing the same indole-ethylamine core but bearing an acetoxy group at the 4-position in place of the free hydroxy group.

Because of its structural similarity to naturally occurring tryptamine alkaloids found in psilocybin-producing fungi, 4-AcO-DMT has been included in forensic, toxicological, and analytical chemistry studies. These often focus on its chromatographic behavior, stability, and relationship to other 4-substituted indole derivatives.

In research settings, 4-AcO-DMT is frequently considered alongside related compounds for comparative studies of:

• LC-MS and GC-MS fragmentation patterns within the 4-acetoxy/4-hydroxy tryptamine family;
• Chromatographic retention and separation under varying stationary phases and mobile-phase compositions;
• Hydrolysis, degradation, and general stability under different storage, pH, and sample-preparation conditions.